|
KMID : 0614619930250010082
|
|
Korean Journal of Gastroenterology
1993 Volume.25 No. 1 p.82 ~ p.89
|
|
|
The Genetic Regulation in Reglonal Difference of Specific Activity of Rat Intestinal Angiotensin converting Enzyme
|
|
|
|
|
Abstract
|
|
|
Recent reports indicate that angiotensin converting enzyme (ACE) in intestinal mucosa may be involved in protein digestion in vivo. The specific activities of intestinal ACE show regional difference. We performed this experiment to define the
level
of
genetic regulation determining the regional difference of ACE specific activities by the relationship between ACE specific activities and ACE mRNA levels.
Small intestines of 3 Wistar rats were removed and divided into 3 segments of equal length. Mucosal homogenate and brush border membrane (BBM) were prep0ared from scraped mucosa and ACE specific activities were measured in both samples by
fluormoetric
method. Total RNA was extracted by guanidinium thiocyanate and isolated by cesium chloride ultracentrifugation. Each 6μg of isolated RNA was transferred to nitrocellulose membrane by dot and slot apparatus Northern hybridization with
radiolabeled
ACF 5
cDNA of mouse was performed and ACE mRNA was detected by autoradiography.
The means of ACE specific activities (μM/μg protein/min) in homogenate and BBM of proximal intestines were 23.4±4.8, 286.3±80.6, in those of middle intestines were 21.3±2.0, 211.0±78.8 and those of distal intestines were 8.2±1.9,
76.4±10.7.
The
means of relative amounts of ACE mRNA in proximal, middle and distal intestines were 75%, 96% and 10% respectively. In linear regression analysis between ACE specific activities of BBM and relative amounts of ACE mRNA, R-value was 0.60 (p<0.05).
We
concluded that transcription was considered as the level of genetic regulation determining the regional difference of ACE specific activities.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
   
|